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mic genome sequence version 2  (Broad Institute Inc)

 
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    Broad Institute Inc mic genome sequence version 2
    Identification of Heterochromatin Body Components (A) A <t>single</t> <t>Tetrahymena</t> thermophila cell possesses a MAC (a) and a <t>MIC</t> (i). During vegetative growth, these nuclei divide and are segregated independently into daughter cells. Nutritional starvation induces the conjugation of two cells carrying different mating types. In the early conjugation stage (∼1–4 hpm), the MICs undergo meiosis. In the mid-stage, one of the meiotic products is exchanged between the cells (∼5 hpm) and fuses with the stationary meiotic product to form a zygotic nucleus (∼6 hpm), which then divides twice to form two new MACs and two MICs (∼7 hpm). At the late-stage, the new MACs (na) are enlarged (∼8 hpm). The pair is dissolved and the parental MAC (pa) and one of the MICs are degraded in the exconjugants (∼12–16 hpm). The exconjugants resume vegetative growth when nutrients are available. (B) Summary of the screen for heterochromatin body components. (C) WT cells at 10, 12, 14, and 16 hpm were hybridized with a probe complementary to the Tlr1 element (green) and stained with an anti-Pdd1p antibody (red). DNA was stained with DAPI (blue). Arrowheads indicate the MIC (i), the new MAC (na), and the parental MAC (pa). The scale bars represent 10 μm. (D) Exconjugants expressing the indicated proteins tagged with EGFP (green) and Pdd1p-mCherry (red) were counterstained with DAPI (blue). Arrowheads indicate the new MACs (na). All pictures share the scale bar, representing 10 μm. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
    Mic Genome Sequence Version 2, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mic+genome+sequence+version+2/pmc04695338-225-1-14?v=Broad+Institute+Inc
    Average 90 stars, based on 1 article reviews
    mic genome sequence version 2 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Phosphorylation of an HP1-like Protein Regulates Heterochromatin Body Assembly for DNA Elimination"

    Article Title: Phosphorylation of an HP1-like Protein Regulates Heterochromatin Body Assembly for DNA Elimination

    Journal: Developmental Cell

    doi: 10.1016/j.devcel.2015.11.017

    Identification of Heterochromatin Body Components (A) A single Tetrahymena thermophila cell possesses a MAC (a) and a MIC (i). During vegetative growth, these nuclei divide and are segregated independently into daughter cells. Nutritional starvation induces the conjugation of two cells carrying different mating types. In the early conjugation stage (∼1–4 hpm), the MICs undergo meiosis. In the mid-stage, one of the meiotic products is exchanged between the cells (∼5 hpm) and fuses with the stationary meiotic product to form a zygotic nucleus (∼6 hpm), which then divides twice to form two new MACs and two MICs (∼7 hpm). At the late-stage, the new MACs (na) are enlarged (∼8 hpm). The pair is dissolved and the parental MAC (pa) and one of the MICs are degraded in the exconjugants (∼12–16 hpm). The exconjugants resume vegetative growth when nutrients are available. (B) Summary of the screen for heterochromatin body components. (C) WT cells at 10, 12, 14, and 16 hpm were hybridized with a probe complementary to the Tlr1 element (green) and stained with an anti-Pdd1p antibody (red). DNA was stained with DAPI (blue). Arrowheads indicate the MIC (i), the new MAC (na), and the parental MAC (pa). The scale bars represent 10 μm. (D) Exconjugants expressing the indicated proteins tagged with EGFP (green) and Pdd1p-mCherry (red) were counterstained with DAPI (blue). Arrowheads indicate the new MACs (na). All pictures share the scale bar, representing 10 μm. See also <xref ref-type=Figure S1 . " title="... thermophila cell possesses a MAC (a) and a MIC (i). During vegetative growth, these nuclei divide and ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Identification of Heterochromatin Body Components (A) A single Tetrahymena thermophila cell possesses a MAC (a) and a MIC (i). During vegetative growth, these nuclei divide and are segregated independently into daughter cells. Nutritional starvation induces the conjugation of two cells carrying different mating types. In the early conjugation stage (∼1–4 hpm), the MICs undergo meiosis. In the mid-stage, one of the meiotic products is exchanged between the cells (∼5 hpm) and fuses with the stationary meiotic product to form a zygotic nucleus (∼6 hpm), which then divides twice to form two new MACs and two MICs (∼7 hpm). At the late-stage, the new MACs (na) are enlarged (∼8 hpm). The pair is dissolved and the parental MAC (pa) and one of the MICs are degraded in the exconjugants (∼12–16 hpm). The exconjugants resume vegetative growth when nutrients are available. (B) Summary of the screen for heterochromatin body components. (C) WT cells at 10, 12, 14, and 16 hpm were hybridized with a probe complementary to the Tlr1 element (green) and stained with an anti-Pdd1p antibody (red). DNA was stained with DAPI (blue). Arrowheads indicate the MIC (i), the new MAC (na), and the parental MAC (pa). The scale bars represent 10 μm. (D) Exconjugants expressing the indicated proteins tagged with EGFP (green) and Pdd1p-mCherry (red) were counterstained with DAPI (blue). Arrowheads indicate the new MACs (na). All pictures share the scale bar, representing 10 μm. See also Figure S1 .

    Techniques Used: Conjugation Assay, Staining, Expressing



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    Identification of Heterochromatin Body Components (A) A <t>single</t> <t>Tetrahymena</t> thermophila cell possesses a MAC (a) and a <t>MIC</t> (i). During vegetative growth, these nuclei divide and are segregated independently into daughter cells. Nutritional starvation induces the conjugation of two cells carrying different mating types. In the early conjugation stage (∼1–4 hpm), the MICs undergo meiosis. In the mid-stage, one of the meiotic products is exchanged between the cells (∼5 hpm) and fuses with the stationary meiotic product to form a zygotic nucleus (∼6 hpm), which then divides twice to form two new MACs and two MICs (∼7 hpm). At the late-stage, the new MACs (na) are enlarged (∼8 hpm). The pair is dissolved and the parental MAC (pa) and one of the MICs are degraded in the exconjugants (∼12–16 hpm). The exconjugants resume vegetative growth when nutrients are available. (B) Summary of the screen for heterochromatin body components. (C) WT cells at 10, 12, 14, and 16 hpm were hybridized with a probe complementary to the Tlr1 element (green) and stained with an anti-Pdd1p antibody (red). DNA was stained with DAPI (blue). Arrowheads indicate the MIC (i), the new MAC (na), and the parental MAC (pa). The scale bars represent 10 μm. (D) Exconjugants expressing the indicated proteins tagged with EGFP (green) and Pdd1p-mCherry (red) were counterstained with DAPI (blue). Arrowheads indicate the new MACs (na). All pictures share the scale bar, representing 10 μm. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
    Mic Genome Sequence Version 2, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Identification of Heterochromatin Body Components (A) A <t>single</t> <t>Tetrahymena</t> thermophila cell possesses a MAC (a) and a <t>MIC</t> (i). During vegetative growth, these nuclei divide and are segregated independently into daughter cells. Nutritional starvation induces the conjugation of two cells carrying different mating types. In the early conjugation stage (∼1–4 hpm), the MICs undergo meiosis. In the mid-stage, one of the meiotic products is exchanged between the cells (∼5 hpm) and fuses with the stationary meiotic product to form a zygotic nucleus (∼6 hpm), which then divides twice to form two new MACs and two MICs (∼7 hpm). At the late-stage, the new MACs (na) are enlarged (∼8 hpm). The pair is dissolved and the parental MAC (pa) and one of the MICs are degraded in the exconjugants (∼12–16 hpm). The exconjugants resume vegetative growth when nutrients are available. (B) Summary of the screen for heterochromatin body components. (C) WT cells at 10, 12, 14, and 16 hpm were hybridized with a probe complementary to the Tlr1 element (green) and stained with an anti-Pdd1p antibody (red). DNA was stained with DAPI (blue). Arrowheads indicate the MIC (i), the new MAC (na), and the parental MAC (pa). The scale bars represent 10 μm. (D) Exconjugants expressing the indicated proteins tagged with EGFP (green) and Pdd1p-mCherry (red) were counterstained with DAPI (blue). Arrowheads indicate the new MACs (na). All pictures share the scale bar, representing 10 μm. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
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    Image Search Results


    Identification of Heterochromatin Body Components (A) A single Tetrahymena thermophila cell possesses a MAC (a) and a MIC (i). During vegetative growth, these nuclei divide and are segregated independently into daughter cells. Nutritional starvation induces the conjugation of two cells carrying different mating types. In the early conjugation stage (∼1–4 hpm), the MICs undergo meiosis. In the mid-stage, one of the meiotic products is exchanged between the cells (∼5 hpm) and fuses with the stationary meiotic product to form a zygotic nucleus (∼6 hpm), which then divides twice to form two new MACs and two MICs (∼7 hpm). At the late-stage, the new MACs (na) are enlarged (∼8 hpm). The pair is dissolved and the parental MAC (pa) and one of the MICs are degraded in the exconjugants (∼12–16 hpm). The exconjugants resume vegetative growth when nutrients are available. (B) Summary of the screen for heterochromatin body components. (C) WT cells at 10, 12, 14, and 16 hpm were hybridized with a probe complementary to the Tlr1 element (green) and stained with an anti-Pdd1p antibody (red). DNA was stained with DAPI (blue). Arrowheads indicate the MIC (i), the new MAC (na), and the parental MAC (pa). The scale bars represent 10 μm. (D) Exconjugants expressing the indicated proteins tagged with EGFP (green) and Pdd1p-mCherry (red) were counterstained with DAPI (blue). Arrowheads indicate the new MACs (na). All pictures share the scale bar, representing 10 μm. See also <xref ref-type=Figure S1 . " width="100%" height="100%">

    Journal: Developmental Cell

    Article Title: Phosphorylation of an HP1-like Protein Regulates Heterochromatin Body Assembly for DNA Elimination

    doi: 10.1016/j.devcel.2015.11.017

    Figure Lengend Snippet: Identification of Heterochromatin Body Components (A) A single Tetrahymena thermophila cell possesses a MAC (a) and a MIC (i). During vegetative growth, these nuclei divide and are segregated independently into daughter cells. Nutritional starvation induces the conjugation of two cells carrying different mating types. In the early conjugation stage (∼1–4 hpm), the MICs undergo meiosis. In the mid-stage, one of the meiotic products is exchanged between the cells (∼5 hpm) and fuses with the stationary meiotic product to form a zygotic nucleus (∼6 hpm), which then divides twice to form two new MACs and two MICs (∼7 hpm). At the late-stage, the new MACs (na) are enlarged (∼8 hpm). The pair is dissolved and the parental MAC (pa) and one of the MICs are degraded in the exconjugants (∼12–16 hpm). The exconjugants resume vegetative growth when nutrients are available. (B) Summary of the screen for heterochromatin body components. (C) WT cells at 10, 12, 14, and 16 hpm were hybridized with a probe complementary to the Tlr1 element (green) and stained with an anti-Pdd1p antibody (red). DNA was stained with DAPI (blue). Arrowheads indicate the MIC (i), the new MAC (na), and the parental MAC (pa). The scale bars represent 10 μm. (D) Exconjugants expressing the indicated proteins tagged with EGFP (green) and Pdd1p-mCherry (red) were counterstained with DAPI (blue). Arrowheads indicate the new MACs (na). All pictures share the scale bar, representing 10 μm. See also Figure S1 .

    Article Snippet: The MIC genome sequence (version 2) was obtained from the Tetrahymena Comparative Sequencing Project (Broad Institute of MIT and Harvard).

    Techniques: Conjugation Assay, Staining, Expressing